RNA ScreenTape Assays for TapeStation Systems from Agilent Technologies

Product Specs
ItemRNA ScreenTape Assays for TapeStation Systems
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog Number5067-5576
Analysis Time16 samples < 20 min
96 samples < 95 min
TypeRNA ScreenTape Assay
Sample TypeEukaryotic or Prokaryotic Total RNA QC
Number of Samples112

Features

Uses per ScreenTape: 16
Quantitative Range: 25 to 500 ng/µL
Sensitivity: 5 ng/µL

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2LSci RepDecember 19, 2023Mirna Barsoum, Roksaneh Sayadi-Boroujeni, Alexander T. Stenzel, Philip Bussmann, Juliane Lüscher-Firzlaff, Bernhard Lüscherisolated as described above. For quality control, samples were analyzed using an RNA ScreenTape (Agilent, 5067-5576) with a TapeStation-device (Agilent). For internal control, ERCC-RNA-spike-in (ThermoFisher, 4456740)

(2) Retinal transcriptome profiling identifies novel candidate genes associated with visual impairment in a mouse model of multiple sclerosisAnimal Cells and SystemsDecember 11, 2023Sungmoo Hong, Poornima D. E. Weerasinghe-Mudiyanselage, Sohi Kang, Changjong Moon, Taekyun Shindetermined using Quant-IT RiboGreen (#R11490, Invitrogen, Carlsbad, CA, USA). TapeStation RNA ScreenTape (#5067-5576, Agilent, Santa Clara, CA, USA) was utilized to assess the integrity of the total RNA, and only high-quality

(3) Uridine–cytidine kinase 2 potentiates the mutagenic influence of the antiviral β-d-N4-hydroxycytidineNucleic Acids ResearchDecember 11, 2023Zhen Xu, Christoffer Flensburg, Rebecca A Bilardi, Ian J MajewskiRNeasy Mini Kit (74104, Qiagen), then quantified using RNA ScreenTape on a 4200 TapeStation System (5067–5576, Agilent). Libraries were prepared using the TruSeq RNA Library Prep Kit v2 (RS-122–2001, RS-122–2002,

(4) Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculatureBrain, Behavior, & Immunity - HealthDecember 1, 2023Marina Wakid, Daniel Almeida, Zahia Aouabed, Reza Rahimian, Maria Antonietta Davoli, Volodymyr Yerko, Elena Leonova-Erko, Vincent Richard, René Zahedi, Christoph Borchers, et al.RNA Purification Kit (Norgen Biotek Corp., cat. no. 51800)•TapeStation RNA ScreenTape (Agilent, cat. no. 5067–5576) or TapeStation High Sensitivity RNA ScreenTape (Agilent, cat. no. 5067–5579)•TapeStation RNA ScreenTape sample buffer

(5) RNA is a key component of extracellular DNA networks in Pseudomonas aeruginosa biofilmsNature CommunicationsNovember 27, 2023Sudarsan Mugunthan, Lan Li Wong, Fernaldo Richtia Winnerdy, Stephen Summers, Muhammad Hafiz Bin Ismail, Yong Hwee Foo, Tavleen Kaur Jaggi, Oliver W. Meldrum, Pei Yee Tiew, Sanjay H. Chotirmall, et al.with double-distilled water. For tape station analysis (Agilent 2200, catalogue numbers: screen tape 5067-5576 and sample buffer 5067-5577), digested samples were centrifuged in situ for 5 min at 3824 x g, the supernatant

(6) Broad-spectrum metastasis suppressing compounds and therapeutic uses thereof in human tumorsScientific ReportsNovember 21, 2023Pnina Gottfried Komlosh, Jonathan L. Chen, Jessica Childs-Disney, Matthew D. Disney, Dan Canaaniintegrity was examined on Tapestation 2200 (Agilent) using RNA Tape and Buffer (Agilent RNA SCREEN TAPE 5067–5576, RNA SCREEN TAPE SAMPLE BUFFER 5067–5577) and confirmed to be > 9.5 for all samples. Poly (A) selection

(7) Adaptive olfactory circuitry restores function despite severe olfactory bulb degenerationCurrent BiologyNovember 20, 2023Tamar Licht, Michael Yunerman, Ido Maor, Naheel Lawabny, Renana Oz Rokach, Idit Shiff, Adi Mizrahi, Dan Roknimanufacturer’s guidelines. ∼200ng of RNA was used for the library construction. RNA ScreenTape kit (catalog #5067-5576; Agilent Technologies, Santa Clara, CA), D1000 ScreenTape kit (catalog #5067-5582; Agilent Technologies),

(8) Unraveling Connective Tissue Growth Factor as a Therapeutic Target and Assessing Kahweol as a Potential Drug Candidate in Triple-Negative Breast Cancer TreatmentInternational Journal of Molecular SciencesNovember 14, 2023Jeong Hee Lee, Jongsu Kim, Hong Sook Kim, Young Jin Kangthe total RNA, samples were run on the TapeStation RNA screentape (Agilent, Santa Clara, CA, USA, #5067-5576). Only high-quality RNA preparations, with RINs greater than 7.0, were used for the RNA library’s construction. A

(9) Extracellular vesicle small RNA cargo discriminates non-cancer donors from pediatric B-lymphoblastic leukemia patientsFrontiers in OncologyNovember 13, 2023Modeline N. Longjohn, Jo-Anna B. J. Hudson, Lourdes Peña-Castillo, Robert P. J. Cormier, Brandon Hannay, Simi Chacko, Stephen M. Lewis, Paul C. Moorehead, Sherri L. ChristianAgilent small RNA kit (Agilent, 5067-1548) and the HS RNA assay on the fragment Analyzer (Agilent, 5067-5576) for integrity and size distribution respectively. 2.10 Library preparation The library was prepared

(10) Next generation sequencing uncovers multiple miRNAs associated molecular targets in gallbladder cancer patientsScientific ReportsNovember 4, 2023Rahul Saxena, Baskar Chakrapani, M. P. Sarath Krishnan, Amit Gupta, Sweety Gupta, Jayanta Das, Subash C. Gupta, Anissa A. Mirza, Shalinee Rao, Bela GoyalRNA quality, and Tape Station was used to analyse RNA integrity using RNA screen Tapes (Agilent, Cat# 5067–5576). RNA library prep and miRNA sequencing NEB Next Multiplex Small RNA Library Prep Kit was utilized

(11) A short peptide protects from age‐onset proteotoxicityAging CellOctober 27, 2023Hassan Elsana, Reut Bruck‐Haimson, Huadong Zhu, Atif Ahmed Siddiqui, Adam Zaretsky, Irit Cohen, Hana Boocholez, Noa Roitenberg, Lorna Moll, Inbar Plaschkes, et al.quantified using a NanoDrop 2000c spectrophotometer. For NGS, we used RNA ScreenTape kit (catalog #5067‐5576; Agilent Technologies), D1000 ScreenTape kit (catalog #5067‐5582; Agilent Technologies), Qubit® RNA HS Assay kit

(12) Spatiotemporal deposition of cell wall polysaccharides in oat endosperm during grain developmentPlant PhysiologyOctober 20, 2023Pavani P Nadiminti, Sarah M Wilson, Allison van de Meene, Alfie Hao, John Humphries, Julian Ratcliffe, Changyu Yi, Marta Peirats-Llobet, Mathew G Lewsey, James Whelan, et al.analyses were undertaken using a 4150 TapeStation System (Agilent) with RNA Screen Tapes (Agilent, 5067-5576) following the manufacturer's instructions. RNA-seq library construction and sequencing Libraries

(13) A myeloid program associated with COVID-19 severity is decreased by therapeutic blockade of IL-6 signalingiScienceOctober 20, 2023Jason A. Hackney, Haridha Shivram, Jason Vander Heiden, Chris Overall, Luz Orozco, Xia Gao, Eugene Kim, Nathan West, Aditi Qamra, Diana Chang, et al.Q32852) and quality was assessed using RNA ScreenTape on 4200 TapeStation (Agilent Technologies catalog: 5067–5576). cDNA library was generated from 2 ng of total RNA using Smart-Seq V4 Ultra Low Input RNA Kit (Takara

(14) Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomesBMC MicrobiologyOctober 20, 2023Asako Tan, Senthil Murugapiran, Alaya Mikalauskas, Jeff Koble, Drew Kennedy, Fred Hyde, Victor Ruotti, Emily Law, Jordan Jensen, Gary P. Schroth, et al.Agilent 4200 TapeStation System using RNA ScreenTape or High Sensitivity RNA ScreenTape (Agilent, cat# 5067–5576 and 5067–5579). RNA integrity numbers (RINs) were highly variable across body sites/sample types and

(15) Identifying key underlying regulatory networks and predicting targets of orphan C/D box SNORD116 snoRNAs in Prader-Willi syndromebioRxivOctober 12, 2023Rachel B. Gilmore, Yaling Liu, Christopher E. Stoddard, Michael S. Chung, Gordon G. Carmichael, Justin Cotneyusing the Agilent TapeStation 4200 with RNA ScreenTape Analysis, including RNA ScreenTape (Agilent, 5067-5576), RNA ScreenTape Sample Buffer (Agilent, 5067-5577), and RNA ScreenTape Ladder (Agilent, 5067-5578).

(16) Methods for cell isolation and analysis of the highly regenerative tunicate Polycarpa mytiligeraFrontiers in Cell and Developmental BiologyOctober 11, 2023Tal Gordon, Noam Hendin, Omri Wurtzelmicrocapillary electrophoresis (TapeStation 4200, G2991BA) with RNA ScreenTape kit (Agilent Technologies, #5067-5576). RNA sequencing library preparation Illumina-sequencing compatible RNAseq libraries were prepared

(17) Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicitynpj Biofilms and MicrobiomesOctober 9, 2023Eva Zanditenas, Meirav Trebicz-Geffen, Divya Kolli, Laura Domínguez-García, Einan Farhi, Liat Linde, Diego Romero, Matthew Chapman, Ilana Kolodkin-Gal, Serge AnkriElectronic Instrument, Petach-Tikva, Israel), along with the RNA ScreenTape & Reagents kit (cat no. 5067-5576). The obtained RINe values for all samples fell within the range of 8.9–9.9, signifying excellent RNA

(18) Large Scale Comparative Deconvolution Analysis of the Canine and Human Osteosarcoma Tumor Microenvironment Uncovers Conserved Clinically Relevant SubtypesbioRxivOctober 6, 2023Sushant Patkar, Josh Mannheimer, Stephanie Harmon, Christina Mazcko, Peter Choyke, G Tom Brown, Baris Turkbey, Amy LeBlanc, Jessica Beckassessed using Nanodrop 8000 (Thermofisher) and Agilent 4200 Tapestation with RNA Screen Tape (Cat# 5067-5576) and RNA Screen Tape sample Buffer (Cat#5067-5577). Between 100ng to 1ug of total RNA was used as the

(19) Phenotypic heterogeneity drives differential disease outcome in a mouse model of triple negative breast cancerFrontiers in OncologySeptember 29, 2023Archana P. Thankamony, Sonny Ramkomuth, Shikha T. Ramesh, Reshma Murali, Priyanka Chakraborty, Nitheesh Karthikeyan, Binitha Anu Varghese, Vishnu Sunil Jaikumar, Mohit Kumar Jolly, Alexander Swarbrick, et al.Invitrogen). RNA purity was determined using QIAxpert and RNA integrity was measured using RNA ScreenTapes (5067-5576, Agilent) on TapeStation. All the 9 RNA samples passed the QC and were used for the library prep. A

(20) mRNA vaccine quality analysis using RNA sequencingNature CommunicationsSeptember 21, 2023Helen M. Gunter, Senel Idrisoglu, Swati Singh, Dae Jong Han, Emily Ariens, Jonathan R. Peters, Ted Wong, Seth W. Cheetham, Jun Xu, Subash Kumar Rai, et al.distribution was evaluated using TapeStation electrophoresis with RNA ScreenTapes (Agilent Technologies, USA, 5067-5576). Detection of dsRNA contaminants by dot blot analysis The IVT mRNA samples were diluted in nuclease-free

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RNA ScreenTape

RNA ScreenTape Assays for TapeStation Systems from Agilent Technologies

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RNA ScreenTape Assays for TapeStation Systems from Agilent Technologies

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